Wanderers lost for the second time in four days after being edged out 1-0 by Yeovil Town at Huish Park.
Ed Upson's 30-yard wonder-strike on eight minutes was the difference between the two sides in what was an evenly-contested encounter in Somerset.
Joel Grant twice went close to pulling the Blues level before the break, while Steven MacLean hit the crossbar and Upson saw an effort cleared off the line by Danny Foster at the other end.
Wycombe's best chance came with 15 minutes remaining when Elliot Benyon's close-range header was expertly saved by Jed Steer, but instead of a late onslaught, the hosts finished strongly and should of extended their lead late on through substitute Gavin Massey.
Gary Waddock made three changes for the clash with the Glovers, recalling Foster, Kevin Betsy and Benyon to the starting XI at the expense of Marvin McCoy, Ben Harding and Stuart Beavon, who missed out with a head injury he picked up in Saturday's 1-0 defeat to Brentford.
There was also no place in the 16-man squad for captain Gareth Ainsworth, who was unavailable because of a back complaint.
After starting the more purposeful of the two sides, Wanderers found themselves behind on eight minutes when Upson fired a 35-yard strike past Nikki Bull into the top left-hand corner.
The boys in blue showed no signs of being affected by the early setback and continued to press their hosts, with Elliot Benyon forcing a smart block from keeper Steer after the striker had been put through on goal by Betsy.
Betsy was looking dangerous on his first start of the league campaign and the Yeovil defence had to be alert to clear his teasing pull-back, while, at the other end, Anthony Edgar blazed an effort over from eight yards.
Wycombe were inches away from drawing level just before the half-hour mark, courtesy of Grant, but the winger sent his rasping low drive just the wrong side of the left-hand post after cutting in from the left touchline.
MacLean clipped the top of the crossbar from 15 yards and Foster cleared Upson's goalbound effort off the line as Yeovil looked to double their lead before the break, but the last chance of the first 45 fell to the lively Grant, who drove his shot just wide.
Betsy curled a 25-yard effort over the top as the Blues pushed for a leveller in the early stages of the second period, but Terry Skiverton's Glovers continued to pose a threat and Edgar wasted a good opportunity shortly afterwards, failing to test Bull from 15 yards after Stuart Lewis had lost his footing.
Scott Rendell replaced Ben Strevens just after the hour mark and with his first touch sent in a teasing cross which had to be turned behind by Luke Ayling.
Wanderers were getting closer, and only a top save from Steer denied Benyon's close-range header finding the back of the net after Andy Sandell's inswinging free-kick had perfectly picked out the striker.
The hosts had a chance to make it 2-0 in the final minutes when on-loan Watford striker Massey was freed down the right, but, after jinking inside James Tunnicliffe, he couldn't beat Bull from 15 yards.
Yeovil did enough to retain possession in the closing stages and deny Wanderers the chance to snatch an equaliser, and Gary Waddock's men must now look to pick themselves up and find a goal when they travel to Tranmere Rovers on Saturday.